Composite

Part:BBa_K2834003:Design

Designed by: Ana Laura Ortega Ceniceros   Group: iGEM18_Tec-Chihuahua   (2018-10-07)


Expressible apidaecin antimicrobial peptide from Apis mellifera


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 86
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the correct expression of this peptide, a chassis with the T7 RNA polymerase gene such as E. coli BL21 (DE3) is needed. An IPTG-inducible promoter was considered for the composite in order to control the expression levels.



Source

This part was designed using basic ones: T7 promoter + RBS (BBa_K525998) + pelB leader sequence (BBa_J32015) + apidaecin (BBa_K2834000) + 6x His-Tag (BBa_K2834002) + T1 terminator from E. coli rrnB (BBa_B0010)

References